Figure 3. Expression of PDL-29 mRNA in rat periodontium. In situ hybridization on periodontal tissue sections from Wistar rat was performed with an anti-sense probe from human PDL-29 cDNA (A,B,C). Dense signals are observed in GFs and PFs. When a sense riboprobe is used, no signal is observed in the tissue (D,E). Neighboring sections were stained with hematoxylin and eosin (F,G). Gingival tissue (B,D,F) and periodontal ligament (C,E,G) correspond to the areas in panel A. Bar equals 300 µm. to, tooth; gt, gingival connective tissue; ab, alveolar bone; pl, periodontal ligament.