Figure 2. CD14 expression on monocytes, TNF- production, and genotypes. (A) Similar CD14 expression on PBMC in each CD14 genotype. The data are expressed as mean ± SEM of 3 replicates. There was no significant difference in the mean fluorescence intensity (unpaired t test). (B) Elevated TNF- production by LPS-stimulated PBMC from TT homozygotes. PBMC were cultured with 1 µg/mL of P. gingivalis LPS for 24 hrs. Culture supernatants were harvested and TNF- levels were determined by ELISA. The data are expressed as mean ± SEM of 4 replicates. TT homozygous was significantly higher than CC homozygous (p = 0.0036) and CT heterozygous (p = 0.0028) (unpaired t test).