Figure 1. Effect of pre-treatment with vehicle, norepinephrine (10 nM), or epinephrine (10 nM) on capsaicin-evoked release of immunoreactive calcitonin gene-related peptide (iCGRP) from in vitro-superfused dental pulp. Bovine dental pulp was collected, sectioned into 200-µm² slices, and superfused with oxygenated Krebs’ buffer. Levels of iCGRP released from dental pulp were collected over seven-minute fractions and measured by radioimmunoassay. The test agents or vehicle was administered from 0-28 min, and capsaicin (10 µM) was administered to all tissues over 21-28 min. Data are calculated as % increase over basal rate of release. Error bars are SEM. **p < 0.01 vs. vehicle. p < 0.01 vs. vehicle and norepinephrine.