Figure 4. (a-c) TUNEL staining with immunostaining one day after injury. In some TUNEL-positive cells (blue signals), signals of p-JNK and p-c-Jun were detected (black arrowheads in a,b). Signals of HSP70 were detected only in TUNEL-negative cells (black arrows in c). (d,e) Double-immunostaining of p-JNK, p-c-Jun, and HSP70 in one day. In some pulp cells, the co-localization of HSP70 with p-JNK (d) and p-c-Jun (e) was observed (yellow signals indicated by white arrows). Pulp cells predominantly expressing HSP70 were also observed (red signals indicated by white arrowheads). Scale bar = 5 µm.