Figure 2. Bmp4 LacZ expression in mesenchyme-derived pulp cells and odontoblasts is absent in Bmp4LacZ^2.4p mice. Sagittal sections from the head region of newborns were stained for β-galactosidase activity. The upper panels are from Bmp4LacZ^2.4p mice, while the lower panels are from Bmp4^lacZneo knock-in mice. Schematic construct maps are displayed in the right panels. LacZ expression patterns in Bmp4^lacZneo knock-in and Bmp4LacZ^2.4p mice are the same in all incisors and molars. Abbreviations: Am, ameloblast; Od, odontoblast; P, pulp cells. Note that the construct used in Bmp4LacZ^knock-in mice contains nuclear localization signal peptide; thus, the expressed β-galactosidase is mainly in the nucleus. The data shown are representative of four separate experiments.